RESUMO
The proteolysis of calmodulin by fungal protease (type XIX) was greatly enhanced in the presence of dGTP and MS2 RNA. Whereas, only moderate proteolytic activation on bacterial proteases (type XXVI) was observed in the presence of MS2 RNA. No appreciable proteolysis of calmodulin by bacterial protease (type IX) was observed. Proteolytic fragments of calmodulin cleaved by fungal protease exhibited unusual low mobility during SDS-polyacrylamide gel electrophoresis. Similar decreased electrophoretic mobility was also noted in the proteolytic fragments of other Ca2(+)-binding proteins including S-100A protein and parvalbumin.
Assuntos
Calmodulina/metabolismo , Endopeptidases/metabolismo , Animais , Caseínas/metabolismo , Bovinos , DNA Viral/farmacologia , Nucleotídeos de Desoxiguanina/farmacologia , Eletroforese , L-Lactato Desidrogenase/metabolismo , Parvalbuminas/metabolismo , Peptídeos/metabolismo , RNA Viral/farmacologia , Proteínas S100/metabolismoRESUMO
The incidence of hemoglobins S and C was determined for infants born to mothers who had recently emigrated to Miami from Haiti. The rates for hemoglobin S trait was 8.0% and C trait 4.7%. These values were higher than those found for American Blacks studied during the same period. Rates for those homozygous for hemoglobin S and doubly heterozygous for hemoglobins S and C are also reported but due to the infrequency with which they occur, the rates are less reliable. These data demonstrate that these abnormal hemoglobins occur in a significant number of Haitians and implies the existence of a considerable number of individuals with sickle cell anemia and hemoglobin SC disease in that country. It is important that a definitive study be done in Haiti to more precisely quantitate the magnitude of the problem so that appropriate health care strategies can be developed.
Assuntos
Hemoglobina C/análise , Hemoglobina Falciforme/análise , População Negra , Feminino , Florida , Haiti/epidemiologia , Haiti/etnologia , Doença da Hemoglobina SC/complicações , Doença da Hemoglobina SC/epidemiologia , Humanos , Incidência , Recém-Nascido , Masculino , Estados Unidos/epidemiologiaRESUMO
Hemoglobino characteristic were investigated in 492 blood specimens collected in a small island community (1,450 inhabitants) in the Bahamas. Using two different methods, abnormal variants were detected in 20.3% of the sample, including genotypes AS, AC, AF(A/HPFH), SC, and SF. Biodemographic evidence suggests that the origin and distribution of the hemoglobinpathies in this population have been influenced by historical migrations and genetic drift. The opportunity for drift has been particularly heightened by the "founder effect," the maintenance of small population size, a relatively endogamous mating structure, and restricted immigration. Health survey results reveal a wide discrepancy between the actual prevalence of abnormal hemoglobins and that indicated by informant reports.
Assuntos
Hemoglobinas Anormais/genética , Bahamas , Frequência do Gene , Genótipo , Humanos , Índias OcidentaisRESUMO
Hemoglobin characteristics were investigated in 492 blood specimens collected in a small island community (1,450 inhabitants) in the Bahamas. Using two different methods, abnormal variants were detected in 20.3 percent of the sample, including genotypes AS, AC,AF(A/HPFH), SC and SF. Biodemographic evidence suggests that the origin and distribution of the hemoglobinopathies in this population have been influenced by historical migrations and genetic drift. The opportunity for drift has been particularly heightened by the "founder effect", the maintainance of small population size, a relatively endogamous mating structure, and restricted immigration. Health survey results reveal a wide discrepancy between the actual prevalence of abnormal hemoglobins and that indicated by informant reports.
